Objective: To explore the effects of Siwu mixture on chemotherapy-induced decline in ovarian reserve function of rat and rat's ovarian granulosa cell apoptosis. Method: Forty female SD rats randomly divided into normal control group

Cisplatin model group

Siwu treatment groups and Premarin treatment group.Each set was 10 female SD rats. Model group

Siwu group and Premarin group were consecutive intraperitoneal injected of cisplatin (2 mg·kg-1·d-1)for 7 days. The normal group was consecutive intraperitoneal injected of the same amount of normal saline for 7 days. Then the normal group and model group were continuous intragastric infusion with pure watergroups(10 mL·kg-1·d-1) for 7 days

Siwu group was continuous intragastric infusion with Siwu mixture(10 mL·kg-1·d-1) for 7 days

and Premarin group was continuous intragastric infusion with premarin(10 mL·kg-1·d-1) for 7 days. Since the first day up

we made vaginal secretion real smear to observe rat's estrous cycle daily. At the experimental 14 day

we weighed and gave anesthesia to rats

to take serum of rats and using radio-immunity method measuring blood follicle-stimulating hormone(FSH)

luteotrophic hormone(LH)

estradiol(E2) and progestins(Pro). We took double side ovarian of rats

with the same side slice for HE dyeing

and imaged micro measurement the particles cell layer maximum thickness at Image Analyzer

then using TUNEL method to detected particles cell apoptosis. Result: Compared with the normal group

the FSH and apoptosis index were elevated in model group rats

the difference statistically significant (P<0.05)

while E2 is decreased. Compared with the model group

FSH and apoptosis index in Siwu group rats were lower

the difference statistically significant (P<0.05)

while the E2 values were rise. Conclusion: Siwu mixture has a certain degree of inhibition effects on ovarian granulosa cell apoptosis in rat after chemotherapy

but cannot afford to completely fix it.