Objective: To select separation and purification technology for total flavonoids from Glycyrrhiza uralensis that have inhibitory action on tyrosinase. Method: With the content of total flavonoids and tyrosinase inhibitory rate as indexes

separation and purification technology of total flavonoids from G. uralensis was optimized

and effect of different separation conditions on tyrosinase inhibitory rate were investigated in the same time. Result: Optimal separation and purification process for total flavonoids was:polyamide was 30~60 mech

pH of sample solution was 6

solid-liquid ratio 32.48∶1(extract of total flavonoids from G. uralensis-polyamide)

the concentration of sample solution was 3.215 g·L-1

desorption solvent was 70% ethanol

desorption velocity was 3 mL·min-1·g-1

and elution volume was 1 BV. Conclusion: This optimized technology had advantages of low cost

high yield

safe

easy to operate.It was suitable for industrial production.