Objective: To establish the method for determination of crocin-Ⅰand crocin-Ⅱ in the Gardenia jasminoides. Method: High performance liquid chromatography was applied on a Dikma Diamonsil C18 (4.6 mm × 250 mm

5 μm) column at temperature 30 ℃ with 0.1% formic acid-acetonitrile as the mobile phase in gradient elution for 30 min. The flow rate was 1.0 mL·min-1

detected wavelength of 440 nm. Result: The sample quality of crocin-Ⅰand crocin-Ⅱwith the peak area showed good linear relationship; the average recoveries were 96.68% (n=6) and 102.94% (n=6). Conclusion: The method is simple

accurate and reproducible; it can provide the basis of rational application

quality control and the standards for G. jasminoides.