MA Bing-zhi, JU Hai, TIAN Xue-feng, et al. Determination of Kaempfero 3, 7----dirhamnopyranoside in from Different Habitats by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(12): 130-131.
MA Bing-zhi, JU Hai, TIAN Xue-feng, et al. Determination of Kaempfero 3, 7----dirhamnopyranoside in from Different Habitats by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(12): 130-131.DOI:
Objective: To develop a HPLC method for the content determination of kaempferol 3
7-O-α-L-dirhamnopyranoside in Woodwardiae unigemmata. Method: HPLC was applied with a DiamonsilTMC18 column by acetonitrile-water (30∶70) as mobile phase. The flow rate was 1.0 mL·min-1. The column temperature was at 25 ℃ and the UV detection wavelength was at 265 nm. Result: The content range of kaempferol 3
7-O-α-L-dirhamnopyranoside from 9 different habats was from 0.05%-0.43%. Conclusion: The method is simple
accurate and specific. It can be used for determination of kaempferol 3