超高液相色谱法测定白芷配方颗粒中欧前胡素和异欧前胡素的含量

毕晓黎; 罗文汇; 胥爱丽; 谭志灿

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超高液相色谱法测定白芷配方颗粒中欧前胡素和异欧前胡素的含量

更新时间：2024-01-04

- 超高液相色谱法测定白芷配方颗粒中欧前胡素和异欧前胡素的含量
- Determination of Imperatorin and Isoimperatorin in Angelicae Dahurica Radix Formula Granules by UPLC
- 中国实验方剂学杂志 2012年18卷第10期页码：140-142
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- 纸质出版日期：2012
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毕晓黎, 罗文汇, 胥爱丽, 等. 超高液相色谱法测定白芷配方颗粒中欧前胡素和异欧前胡素的含量[J]. 中国实验方剂学杂志, 2012,18(10):140-142.

BI Xiao-li, LUO Wen-hui, XU Ai-li, et al. Determination of Imperatorin and Isoimperatorin in Angelicae Dahurica Radix Formula Granules by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 140-142.
毕晓黎, 罗文汇, 胥爱丽, 等. 超高液相色谱法测定白芷配方颗粒中欧前胡素和异欧前胡素的含量[J]. 中国实验方剂学杂志, 2012,18(10):140-142. DOI：

BI Xiao-li, LUO Wen-hui, XU Ai-li, et al. Determination of Imperatorin and Isoimperatorin in Angelicae Dahurica Radix Formula Granules by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 140-142. DOI：

摘要

目的: 建立同时测定白芷配方颗粒中欧前胡素和异欧前胡素的超高效液相方法。方法: 采用超高效液相色谱法

Thermo Hypersil GOLD C18色谱柱(2.1 mm×100 mm

1.9 μm)柱

流动相甲醇-水(55∶45)

流速0.2 mL·min-1

柱温30 ℃

检测波长300 nm。结果: 欧前胡素在7.8~70.2 ng呈良好的线性关系(r＝0.999 8)

平均回收率为98.82%

RSD 1.25%;异欧前胡素在5.04~45.36 ng呈良好的线性关系(r＝0.999 7)

平均回收率为100.14%

RSD 1.16%。结论: 以建立的方法简便准确、快速、重复性好

可作为有效的方法对白芷配方颗粒中所含欧前胡素和异欧前胡素进行含量测定。

Abstract

Objective: To establish a method for determination of imperatorin and isoimperatorin in Angelicae Dahurica by UPLC. Method: The content of Imperatorin and isoimperatorin was determined with UPLC in this preparation. The Thermo Hypersil GOLD C18(2.1 mm×100 mm

1.9 μm) column was used. The mobile phase consisted of methanol-water (55∶45)solution. The flow rate was 0.2 mL·min-1 and column temperature was at 30 ℃. The detecting wave length was at 300 nm Result: The two components had a good linearity relationship between 7.8-70.2 ng;5.04-45.36 ng.The average recoveries(n=6) were 98.82%(RSD 1.25)

100.14%(RSD 1.16). Conclusion: The methods is simple

accurate

rapid and reproducible

it can be used as an effectively method for the determination of imperatorin and isoimperatorin in Angelicae Dahurica.

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