一测多评法结合指纹图谱对杜仲质量控制的研究

林芳; 王云红; 万丽; 杨荣平

您当前的位置：

首页 >

文章列表页 >

一测多评法结合指纹图谱对杜仲质量控制的研究

更新时间：2024-01-04

- 一测多评法结合指纹图谱对杜仲质量控制的研究
- Study on the Quality Control of Eucommiae Cortex by Multi-components Quantitation by One Marker Method and Fingerprint
- 中国实验方剂学杂志 2012年18卷第13期页码：78-82
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金青年基金项目(30801546)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
移动端阅览
林芳, 王云红, 万丽, 等. 一测多评法结合指纹图谱对杜仲质量控制的研究[J]. 中国实验方剂学杂志, 2012,18(13):78-82.

LIN Fang, WANG Yun-hong, WAN Li, et al. Study on the Quality Control of Eucommiae Cortex by Multi-components Quantitation by One Marker Method and Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 78-82.
林芳, 王云红, 万丽, 等. 一测多评法结合指纹图谱对杜仲质量控制的研究[J]. 中国实验方剂学杂志, 2012,18(13):78-82. DOI：

LIN Fang, WANG Yun-hong, WAN Li, et al. Study on the Quality Control of Eucommiae Cortex by Multi-components Quantitation by One Marker Method and Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 78-82. DOI：

摘要

目的: 建立杜仲多指标HPLC指纹图谱

并应用一测多评法对药材中松脂醇二葡萄糖苷(PDG)、绿原酸(CA)、京尼平苷酸(PA)的含量同时进行测定。方法: 采用WelchromTM C18 110A (4.6 mm×250 mm

5 μm)色谱柱

以甲醇-0.3%冰醋酸进行梯度洗脱

柱温20℃

流速1 mL·min-1

检测波长238 nm

以松脂醇二葡萄糖苷、绿原酸、京尼平苷酸和京尼平苷(GP)为指标性成分建立杜仲药材的指纹图谱。以绿原酸为参照内标

通过相对校正因子对松脂醇二葡萄糖苷、绿原酸、京尼平苷酸进行含量测定。结果: 建立了杜仲多指标HPLC指纹图谱

标定了12个共有指纹峰。对杜仲中3个成分用一测多评法进行了测定

其计算值与外标法实测值之间没有明显差异。结论: 建立了杜仲药材多指标HPLC指纹图谱和一测多评法对松脂醇二葡萄糖苷、绿原酸、京尼平甘酸进行同时测定的方法

该方法简便可行

为更全面合理科学地对杜仲药材质量的评价提供了技术支撑。

Abstract

Objective: To establish the multi-indicator HPLC fingerprint of Eucommiae Cortex and to determine the contents of geniposidic acid

chlorogenic and acid

pinoresinol diglucoside with quantitative analysis of multi-components by single marker. Method: WelchromTM C18 110A (4.6 mm×250 mm

5 μm) column was used

the mobile phase was consisted of methanol-water-0.3% acetic acid as gradient elution. The column temperature was at 20℃.The flow rate was 1.0 mL·min-1 and the fingerprint was detected at 238 nm.Geniposidic acid and chlorogenic acid

pinoresinol diglucoside and geniposide was choosen as marks of ingredients to establish HPLC fingerprint. Using chlorogenic acid as internal reference standard

the contents of geniposidic acid

chlorogenic acid

pinoresinol diglucoside was determined according to the relative correction factor. Result: All tested samples contained the 12 common peaks and there was no significant differences between the quantitative results of three ingredients of quantitative analysis of multi-components by single marker method and external standard method. Conclusion: The multi-indicator HPLC fingerprint and simultaneous determination of geniposidic acid and chlorogenic acid

pinoresinol diglucoside with quantitative analysis of multi-components by single marker were firstly studied.This method was simple and feasible

which would provide better technique support for the quality control of Eucommiae Cortex.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

杜仲及其有效成分治疗膝骨关节炎的作用机制研究现状

经典名方中杜仲的本草考证

基于大鼠离体外翻肠囊模型考察杜仲提取物在正常和自发性高血压状态下的肠吸收特性差异

杜仲及盐杜仲饮片的均匀化分析

一测多评法同时测定附子中5种水溶性成分的含量