GAO Xiu-juan, MA Hui-xia, YAO Rong-mei, et al. Inhibition of Qiaojing Bushen Capsule on Intestinal Glucose Absorption [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 214-216.
GAO Xiu-juan, MA Hui-xia, YAO Rong-mei, et al. Inhibition of Qiaojing Bushen Capsule on Intestinal Glucose Absorption [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 214-216.DOI:
目的: 观察荞精补肾胶囊(QJBS)体外抑制小肠葡萄糖吸收的作用。 方法: SD大鼠禁食不禁水24 h
处死
制备小肠离体外翻模型
随机分为空白对照组、阿卡波糖对照组(0.25 g·L-1)和QJBS 1~QJBS 5 (2.5
5.0
10.0
20.0
40.0 g·L-1)。将肠囊分别放入等量的营养液中
加入麦芽糖及受试药物
在37 ℃水浴箱中孵育120 min
测定葡萄糖浓度并计算抑制率。 结果: QJBS各组对离体小肠葡萄糖的吸收具有抑制作用
当药物质量浓度为20.0 g·L-1
抑制作用最明显
抑制率为(67.7±10.5)%
与阿卡波糖抑制作用相当。 结论: QJBS能够抑制小肠对葡萄糖的吸收
其作用可能与抑制肠道α-糖苷酶类有关。
Abstract
Objective: To observe inhibition of Qiaojing Bushen capsules(QJBS) in vitro on glucose absorptionin small intestinal. Method : SD rats were fasted and gave water for 24 hours
then sacrificed to prepare small intestine in vitro everted model. Rats were divided into the blank control group
and acarbose tablets group 0.25 g·L-1 and QJBS group: QJBS 1-QJBS 5(2.5
5.0
10.0
20.0
40.0 g·L-1). The sugar substrate and tested drugs were added into gut sac
which were incubated for 120 min at 37 ℃ to determin glucose concentrations
and the inhibition rate was calculated. Result: Each group of QJBS can inhibit small intestinal absorption of glucose
when the drug concentration was 20 g·L-1
inhibitory effect was the most obvious. The inhibition rat was (67.7±10.5)%
which was equal to acarbose. Conclusion: QJBS can inhibit intestinal glucose absorption
the effect may be related to inhibition of intestinal α-glucosidase.