Objective: To establish a method to determine the content of desmethoxymatteucinol in Rhizoma Dryopteridis Crassirhizomatis from different areas of China. Method: The analysis was performed on a column of YWG-C18 (4.6 mm×250 mm

10 μm)

using methanol and water (78∶22) as mobile phase and the flow rate was at 1.0 mL·min-1 and detected at 298 nm under 25 ℃. Result: The linearity of calibration curve was in the range of 0.188 0-1.316 μg(r=0.999 7).The average recovery was 99.38%

RSD 2.40%. The highest content of desmethoxymatteucinol in Rhizoma Dryopteridis Crassirhizomatis from Shijiazhuang is 0.35%. The lowest content of desmethoxymatteucinol in Rhizoma Dryopteridis Crassirhizomatis from Xicheng district of Beijing is 0.17%. Conclusion: This method is accurate

repatable and can be applied to determine the content of desmethoxymatteucinol in crude drug Rhizoma Dryopteridis Crassirhizomatis.