PAN Li, LIAO Hou-zhi. Determination of Luteolin in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 145-147.DOI:
Objective: To establish the determination method of luteolin content in Codariocalyx motorius. Method: VP-ODS Cl8(4.6 mm×250 mm
5 μm) colmnn was used
the mobile phase was methanol-0.3% phosphonic acid solution (60∶40) with the flow rate as 1.0 mL·min-1. The detection wavelength was set at 350 nm. Result: The linear relation of hyperin was good in the range from 0.040 72 to 0.651 52 μg. The average recovery was 100.35%
RSD was 0.80%. Conclusion: The method established is accurate
sensitive and good in reproducibility. It can be applied to the quality control of C. motorius.