Objective: To optimize extraction process of dihydromyricetin from Ampelopsis grossedentata by response surface methodology. Method: With extraction ratio of dihydromyricetin as index

based on single factor test

the concentration of ethanol

liquid-solid ratio and extraction time were selected as independent variables and extraction rate of dihydromyricetin as response value

response surface methodology with three-factors and three-levels was carried out for optimizing extraction process of dihydromyricetin from A. grossedentata. Result: Optimum extraction conditions of dihydromyricetin were as followed:refluxing extracted 95 min with 17 times the amount of 81% ethanol. Under these conditions

extraction ratio of dihydromyricetin was 9.58%

relative error in comparison with the predicted one was 0.14%. Conclusion: This optimized technology was reliable

it showed that was feasible for optimizing extraction technology of dihydromyricetin by response surface methodology.