Objective: An HPLC method was established for determination of scopoletin and scopolin in Lycium barbarum. Method: The chromatographic separation was performed on Diamonsil C8 column (4.6 mm×150 mm

5 μm)

acetonitrile-water-acetic acid ( 5:94.5:0.5 ) as mobile phase A and acetonitrile-water-acetic acid (72:27.5:0.5) as mobile phase B with gradient elution. The flow rate was 1 mL·min-1 at 30℃

and the detection wavelength was set at 346 nm. Result: The calibration curve was linear in the range of 0.1-10 mg·L-1 for scopoletin (r=0.9999)

and the regression equation was Y=32568X+637.8.The recovery was 98.2%

with RSD of 1.45%. The calibration curve was linear in the range of 0.1-10 mg·L-1 for scopolin (r=0.9999)

and the regression equation was Y=23951X+746.7.The recovery was 101.6%

with RSD of 1.27%. Conclusion: The method is accurate and reliable

which could be used as a quality control method for scopoletin and scopolin in L. barbarum

and provide basis for development and utilization of L. barbarum.