Objective: To establish a method to determine the pharmacokinetics of 3

6'-disinapoyl sucrose and pharmacokinetic differences of 3

6'-disinapoyl sucrose following oral administration of pure 3

6'-disinapoyl sucrose

Radix Polygalae extract and Kaixinsan were investigated in acquired dysmnesia model rats with approximately the same dose of 3

6'-disinapoyl sucrose. Method: The rats were received injection of scopolamine to make the model of acquired dysmnesia

and orally treat with pure 3

6'-disinapoyl sucrose

Radix Polygalae extract and Kaixinsan respectively. Blood samples were collected via abdominal aorta. Serum samples were immediately separated by centrifugation at 4 000 r·min-1 for 10 min

and 0.1 mmol monopotassium phosphate and acetonitrile were added to deposit proteins. After centrifugation

the upper liquid evaporated to dryness by N2 at 50 ℃. The residue was added distilled water to dissolve and the concentation of 3

6'-disinapoyl sucrose was determined by HPLC. Result: A good linear relationship of 3

6'-disinapoyl sucrose was obtained from 0.052-2.08 mg·L-1

the lowest limits of determination were 52 μg·L-1. The pharmacokinetic parameters were estimated by non-compartmental methods using the Kinetica 4.4 program

and the pharmacokinetic parameters AUC0~∞

Cmax of 3

6'-disinapoyl sucrose showed significant differences between 3

6'-disinapoyl sucrose and other groups. Conclusion: The method applied for determination of 3

6'-disinapoyl sucrose content in blood was simple

feasible and accurate for the study of 3

6'-disinapoyl sucrose pharmacokinetics in rats. The results indicated the other components of Radix Polygalae and compatibility had remarkable influence on the pharmacokinectics of 3

6'-disinapoyl sucrose.