Objective: To optimize enrichment and purification technology of total flavonoids from Leontopodium leontopodioid by macroporous resin. Method: Optimum macroporous resin and its purification technology was optimized by static adsorption/desorption and dynamic adsorption/desorption.The content of total flavonoids was determined by ultraviolet and visible spectrophotometry method. Result: HPD100 macroporous resin showed good concentration and purification capabilities to total flavonoids from L. leontopodioid. Optimum technology conditions were as follows: 2 BV sample liquid (the concentration of raw drug was 0.1 g·mL-1) of L. leontopodioid was passed through HPD-100 resin in 2 h with adsorption rate of 1 BV·h-1

eluted by 5 BV water

4 BV 30% ethanol

3 BV 50% ethanol

respectively

elution flow rate was 4 BV·h-1.Eluate was collected to concentrating under vacuum.Under this optimized technology

the elution rate of total flavonoids was more than 90%. Conclusion: This optimized technology was stable

feasible

it was suitable for industrial production.