Objective: This paper presents the effects of anti simian immunodeficiency virus(SIV) of the aqueous extract from Ganodorma lucidumspores(AEGLS)by using SIVmac and CEM×174 cells as a model in vitro

and the mechanism were preliminarily studied. Method: The inhibitory effects of AEGLS on SIV were observed when SIV and CEM×174 system were employed as the models

and the mechanism of action were preliminarily studied by using time course assay

effective stage analysis

Western blot. Result: AEGLS had a remarkable anti-SIV activity. The IC50 (50% inhibitory concentration) of AEGLS was (466.62±20.21)mg·L-1

and it was low cytotoxicity which its CC50 (50% cellular cytotoxicity) was (3.13±0.11)g·L-1 and selection index was 6.71.The results of anti-SIV mechanism showed AEGLS main acted on the early stage of SIV infection

and might contribute to affect the SIV attachment and/or penetration into the cells. The results of Western blot showed AEGLS could decrease SIV P27 capsid protein expression level. Conclusion: AEGLS had a remarkable anti-SIV activity and is main acted on the early stage of SIV infection

and might contribute to affect the SIV attachment and/or penetration into the cells

and could decrease SIV P27 capsid protein expression level.