透析法制备低相对分子质量人参糖肽的工艺及其性质考察

王颖; 陈英红; 罗浩铭; 姜瑞芝

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透析法制备低相对分子质量人参糖肽的工艺及其性质考察

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- 透析法制备低相对分子质量人参糖肽的工艺及其性质考察
- Investigation of Preparation Technology and Property for Low Molecular Weight Glycopeptide from by Dialysis Method
- 中国实验方剂学杂志 2012年18卷第18期页码：14-16
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81173532);吉林省科技发展计划项目(201201086)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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王颖, 陈英红, 罗浩铭, 等. 透析法制备低相对分子质量人参糖肽的工艺及其性质考察[J]. 中国实验方剂学杂志, 2012,18(18):14-16.

WANG Ying, CHEN Ying-hong, LUO Hao-ming, et al. Investigation of Preparation Technology and Property for Low Molecular Weight Glycopeptide from by Dialysis Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 14-16.
王颖, 陈英红, 罗浩铭, 等. 透析法制备低相对分子质量人参糖肽的工艺及其性质考察[J]. 中国实验方剂学杂志, 2012,18(18):14-16. DOI：

WANG Ying, CHEN Ying-hong, LUO Hao-ming, et al. Investigation of Preparation Technology and Property for Low Molecular Weight Glycopeptide from by Dialysis Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 14-16. DOI：

摘要

目的: 探讨透析法制备低相对分子质量人参糖肽的工艺

并对其理化性质、纯度与相对分子质量分布进行研究。方法: 以理化性质及收率为考察指标

单因素试验对透析制备低相对分子质量人参糖肽的工艺进行考察

采用HPLC及GPC软件测定纯度与分子量分布。结果: 制备工艺确定为采用截流相对分子质量1 000 Da透析袋

加入6 L水透析4 h。制备的人参糖肽总糖肽质量分数92.49%

收率18.95%

确定为相对分子质量在300-3 500 Da的混合物。结论: 采用透析法制备低相对分子质量人参糖肽操作简便易行

总糖肽含量较高

相对分子质量较低。

Abstract

Objective: To investigate preparation process of low molecular weight glycopeptide from Panax ginseng by dialysis method

and study on its physical and chemical properties

purity and molecular weight distribution. Method: With physical and chemical properties and yield as indexes

preparation technology of low molecular weight glycopeptide from P. ginseng by dialysis was investigated by single-factor test

purity and molecular weight distribution were determined by HPLC and GPC software. Result: Preparation process was:closure molecular weight of dialysis bag 1 000 Da

added 6 L water and dialysis time 4 h.The content of total glycopeptide from P. ginseng was 92.49%

yield was 18.95%

it was a mixture with molecular weight 300-3 500 Da. Conclusion: This preparation technology of dialysis was simple

the content of total glycopeptide was high

and molecular weight was low.

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