响应面分析法优化湿法超微粉碎地龙蛋白的提取工艺

杨丰云; 付廷明; 郭立玮; 刘峰; 张伟

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响应面分析法优化湿法超微粉碎地龙蛋白的提取工艺

更新时间：2024-01-04

- 响应面分析法优化湿法超微粉碎地龙蛋白的提取工艺
- Optimization of Extraction Technology for Wet Ultrafine Grinding Technology on Earthworm Protein by Response Surface Methodology
- 中国实验方剂学杂志 2012年18卷第10期页码：33-37
- 作者机构：
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- 基金信息：
  
  "重大新药创制"科技重大专项(2011ZX09401-308-008);江苏省中医药局科技项目(LZ09007)
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- 纸质出版日期：2012
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杨丰云, 付廷明, 郭立玮, 等. 响应面分析法优化湿法超微粉碎地龙蛋白的提取工艺[J]. 中国实验方剂学杂志, 2012,18(10):33-37.

YANG Feng-yun, FU Ting-ming, GUO Li-wei, et al. Optimization of Extraction Technology for Wet Ultrafine Grinding Technology on Earthworm Protein by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 33-37.
杨丰云, 付廷明, 郭立玮, 等. 响应面分析法优化湿法超微粉碎地龙蛋白的提取工艺[J]. 中国实验方剂学杂志, 2012,18(10):33-37. DOI：

YANG Feng-yun, FU Ting-ming, GUO Li-wei, et al. Optimization of Extraction Technology for Wet Ultrafine Grinding Technology on Earthworm Protein by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 33-37. DOI：

摘要

目的: 优选采用湿法超微粉碎法提取地龙蛋白的工艺。方法: 在单因素试验基础上

以浸泡时间、料液比、提取温度、粉碎时间为影响因素

蛋白质得率为响应值

根据中心组合试验设计原理采用四因素三水平的响应面分析法

对湿法超微粉碎提取地龙蛋白条件进行优化。结果: 湿法超微粉碎提取地龙蛋白的最佳工艺条件为浸泡时间4.7 h

料液比1∶11

提取温度17 ℃

粉碎时间10 min。在此条件下蛋白质得率3.46%。结论: 湿法超微粉碎提取技术适用于地龙蛋白提取。该试验优选提取工艺稳定可行

适于工业化推广。

Abstract

Objective: To optimize extraction technology of earthworm protein by wet ultrafine grinding technology. Method: On the basis of single-factor test

extraction technology of wet ultrafine grinding method on earthworm protein was optimized with soaking time

solid-liquid ratio

extraction temperature and grinding time as influencing factors

yield of protein as response value.According to central composite test design principles

4 factors and 3 levels of response surface analysis method was used. Result: Optimum extraction process of earthworm protein by wet ultrafine grinding technology was as follows:soaked 4.7 h

solid-liquid rate 1∶11

extraction temperature 17 ℃

grinding time 10 min. Under these conditions

yield of protein could be up to 3.46%. Conclusion: Wet ultrafine grinding technology was suitable for extraction of earthworm protein.This optimize extraction technology was stable

feasible and suitable for large-scale promotion.

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