Objective: To compare the contents of epiberberine

coptisine

berberine and palmatine in different processed of Coptis chinensis. Method: Quantitative analysis multi-components by single marker was used to determine the the contents of four alkaloids in different processed C. chinensis.Chromatographic conditions were:Agilent TC-C18 column(2) (4.6 mm×250 mm

5 μm)

mobile phase of acetonitrile-0.05 mol · L-1 potassium dihydrogen phosphate(50: 50

plus 0.4 g sodium dodecyl sulfate per 100 mL

phosphate-regulating pH 4.0)

detection wavelength 345 nm

column temperature 25℃

flow rate 1 mL · min-1

number of theoretical plates calculated to berberine hydrochloride should be greater than 2 000. Result: The contents of epiberberine

coptisine

berberine and palmatine in different processed of C. chinensis had different degrees of reduction. Conclusion: Established determination method in this test was rapid and economical

it could be used as quality testing method for different processed of C. chinensis.