毛细管电泳法测定千柏鼻炎片中盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱的含量

丁佳佳; 李征征; 宋粉云

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毛细管电泳法测定千柏鼻炎片中盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱的含量

更新时间：2024-01-04

- 毛细管电泳法测定千柏鼻炎片中盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱的含量
- Determination of Dphedrine Hydrochloride, Pseudoephedrine Hydrochloride and Methylephedrine Hydrochloride in Qianbai Biyan Tabelt by CE
- 中国实验方剂学杂志 2012年18卷第10期页码：120-123
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省自然科学基金项目(5002841)
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  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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丁佳佳, 李征征, 宋粉云. 毛细管电泳法测定千柏鼻炎片中盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱的含量[J]. 中国实验方剂学杂志, 2012,18(10):120-123.

DING Jia-jia, LI Zheng-zheng, SONG Fen-yun. Determination of Dphedrine Hydrochloride, Pseudoephedrine Hydrochloride and Methylephedrine Hydrochloride in Qianbai Biyan Tabelt by CE[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 120-123.
丁佳佳, 李征征, 宋粉云. 毛细管电泳法测定千柏鼻炎片中盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱的含量[J]. 中国实验方剂学杂志, 2012,18(10):120-123. DOI：

DING Jia-jia, LI Zheng-zheng, SONG Fen-yun. Determination of Dphedrine Hydrochloride, Pseudoephedrine Hydrochloride and Methylephedrine Hydrochloride in Qianbai Biyan Tabelt by CE[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 120-123. DOI：

摘要

目的: 建立测定千柏鼻炎片中盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱含量的毛细管电泳法。方法: 采用未涂层弹性融硅石英毛细管柱(67 cm×75 μm

有效长度60 cm)

以130 mmol·L-1 Tris-HCl-60%(体积分数)甲醇(pH 9.27) 为运行缓冲液

分离电压20 kV

重力进样10 s(高度15 cm)

检测波长210 nm。以盐酸氯丙那林为内标。结果: 盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱浓度分别在42.9~92.4

2.4~57.6

1.65~26.4 mg·L-1线性关系良好

3种有效成分的平均加样回收率依次为99.7%

98.4%

95.6%

方法精密度RSD依次为2.70%

2.76%

2.52%(n=6)。结论: 方法简便、快速

结果准确可靠

可用于千柏鼻炎片中的质量控制。

Abstract

Objective: To establish the method for determinnation of ephedrine hydrochloride、pseudoephedrine hydrochloride and methylephedrine hydrochloride in Qianbai Biyan Pian by capillary elctrophoresis. Method: The separation was performed on a fused silica capillary of 67 cm×75 μm (60 cm of effective elength). 130 mmol·L-1Tris-HCl-60% CH3OH(pH 9.27) was selected as the running buffer. The separation voltage was 20 kV. The samples was injected by gravity(10 s

15 cm). The detection wavelength was 210 nm. Result: The linear range of determination for ephedrine hydrochloride、pseudoephedrine hydrochloride and methylephedrine hydrochloride were 42.9-92.4

2.4-57.6

1.65-26.4 mg·L-1. The average recoveries were 99.7%

98.4%

95.6%

precisions of the method were 2.70%

2.76% and 2.52%(RSD

n=6). Conclusion: The method is convenient

rapid

accurate for the quality control of Qianbai Biyan Pian.

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