HPLC测定五味渣驯丸中羟基红花黄色素A、没食子酸和马兜铃酸A的含量

倪琳; 杨锡

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HPLC测定五味渣驯丸中羟基红花黄色素A、没食子酸和马兜铃酸A的含量

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- HPLC测定五味渣驯丸中羟基红花黄色素A、没食子酸和马兜铃酸A的含量
- Determination of Hydroxysafflor Yellow A, Gallic Acid and Aristolochic Acid A in Wuwei Zhaxun Pill by HPLC
- 中国实验方剂学杂志 2012年18卷第18期页码：109-112
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- 纸质出版日期：2012
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倪琳, 杨锡. HPLC测定五味渣驯丸中羟基红花黄色素A、没食子酸和马兜铃酸A的含量[J]. 中国实验方剂学杂志, 2012,18(18):109-112.

NI Lin, YANG Xi. Determination of Hydroxysafflor Yellow A, Gallic Acid and Aristolochic Acid A in Wuwei Zhaxun Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 109-112.
倪琳, 杨锡. HPLC测定五味渣驯丸中羟基红花黄色素A、没食子酸和马兜铃酸A的含量[J]. 中国实验方剂学杂志, 2012,18(18):109-112. DOI：

NI Lin, YANG Xi. Determination of Hydroxysafflor Yellow A, Gallic Acid and Aristolochic Acid A in Wuwei Zhaxun Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 109-112. DOI：

摘要

目的: 建立五味渣驯丸中羟基红花黄色素A、没食子酸、马兜铃酸A的含量测定方法。方法: 采用HPLC对制剂中主要成分羟基红花黄色素A、没食子酸、马兜铃酸A进行定量分析。CAPCELL PAK C18色谱柱(4.6 mm×250 mm

5 μm)

甲醇-0.4%磷酸(30:70)、甲醇-0.2%磷酸(7:93)、甲醇-0.05%冰醋酸(79:21)为流动相

流速1.0 mL ·min-1

检测波长为403

273

390 nm。结果: 羟基红花黄色素A、没食子酸、马兜铃酸A的线性范围分别为0.116~1.740 μg(r=0.999 97)

0.067~0.673 μg(r=0.999 95)

0.024~0.247 μg(r=0.999 99);平均回收率分别为98.62%(RSD 2.15%)

100.01%(RSD 2.03%)

96.83%(RSD 1.74%)。结论: 本方法简便、结果准确可靠

可有效控制制剂质量。

Abstract

Objective: To establish an method for determination of hydroxysafflor yellow A

gallic acid and aristolochic acid A in Wuwei Zhaxun pill. Method: The hydroxysafflor yellow A

gallic acid and aristolochic acid A in Wuwei Zhaxun pill was determined by HPLC. CAPCELL PAK C18(4.6 mm×250 mm

5 μm)column was used and detection wavelength was at 403

273

390 nm. The mobile phase was methanol-0.4% phosphoric acid (30: 70)

methanol-0.2%phosphoric acid (7: 93)

methanol-0.05% glacial acetic acid (79: 21)

with the flow rate of 1.0 mL · min-1.Result: The calibration curves of hydroxysafflor yellow A

gallic acid and aristolochic acid A were in a good linearity over the ranges of 0.116-1.740 μg (r=0.999 97)

0.067-0.673 μg(r=0.999 95)

0.024-0.247 μg(r=0.999 99)

respectively. The average recoveries were 98.62%(RSD 2.15%)

100.01%(RSD 2.03%)

96.83%(RSD 1.74%). Conclusion: The method is simple

accurate and can be used for the control.

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