Objective: To establish a HPLC method for the determination of eldrin

quertetin and kaempferol in Impatiens balsamina. Method: The determination was performed on a Diamonsil C18 column(4.6 mm×250 mm

5 μm)

with a gredient mobile phase of acetonitrile-0.1% glacial acetic acid;the flow rate was 1.0 mL · min-1;column temperature was 30℃;the detection wavelength was at 260 nm. Result: The chromatographic peaks of eldrin

quertetin and kaempferol with other components were completely separated.The average recovery was 98.71%

98.96%

98.89%

RSD 2.13%

2.34%

2.38%. Conclusion: The method was rapid and accurate for quality control of I. balsamina

and laid foundations for further research and development.