免疫亲和柱净化HPLC柱后光化学衍生化法检测中药及染菌中药制剂中间体的黄曲霉毒素

胡一晨; 万丽; 范成杰; 吕维; 杨芳; 吉琅

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免疫亲和柱净化HPLC柱后光化学衍生化法检测中药及染菌中药制剂中间体的黄曲霉毒素

更新时间：2024-01-04

- 免疫亲和柱净化HPLC柱后光化学衍生化法检测中药及染菌中药制剂中间体的黄曲霉毒素
- Investigation on Detection of Aflatoxins in Chinese Materia Medica and Pharmaceutical Intermediates Microbiological Contaminated by Immunoaffinity Column Clean-up Combined with Post-column Derivatization
- 中国实验方剂学杂志 2012年18卷第10期页码：116-119
- 作者机构：
- 作者简介：
- 基金信息：
  
  四川省教育厅科技创新重大培育项目(09ZZ08)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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胡一晨, 万丽, 范成杰, 等. 免疫亲和柱净化HPLC柱后光化学衍生化法检测中药及染菌中药制剂中间体的黄曲霉毒素[J]. 中国实验方剂学杂志, 2012,18(10):116-119.

HU Yi-chen, WAN Li, FAN Cheng-jie, et al. Investigation on Detection of Aflatoxins in Chinese Materia Medica and Pharmaceutical Intermediates Microbiological Contaminated by Immunoaffinity Column Clean-up Combined with Post-column Derivatization[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 116-119.
胡一晨, 万丽, 范成杰, 等. 免疫亲和柱净化HPLC柱后光化学衍生化法检测中药及染菌中药制剂中间体的黄曲霉毒素[J]. 中国实验方剂学杂志, 2012,18(10):116-119. DOI：

HU Yi-chen, WAN Li, FAN Cheng-jie, et al. Investigation on Detection of Aflatoxins in Chinese Materia Medica and Pharmaceutical Intermediates Microbiological Contaminated by Immunoaffinity Column Clean-up Combined with Post-column Derivatization[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 116-119. DOI：

摘要

目的: 采用免疫亲和柱净化

结合柱后光化学衍生化的高效液相色谱-荧光检测器建立同步检测常用中药材及染菌中药制剂中间体中的黄曲霉毒素B1

G2的方法。方法: 采用免疫亲和柱净化洗脱结合柱后光化学衍生手段

建立黄曲霉毒素B1

G2的分析方法

并分别对14种中药材及染菌中药制剂中间体进行检测。结果: 黄曲霉毒素B2和G2

B1和G1分别在0.15~6.0和0.5~20.0 ng·mL-1线性关系良好

方法准确稳定。所选的14种中药中川芎和柏子仁检测出黄曲霉毒素B1

而以染菌中药川芎所制备的5种提取物中均未检出黄曲霉毒素。结论: 采用此方法检测常用中药材及其制剂中间体中的黄曲霉毒素无干扰性杂峰

结果准确可靠。

Abstract

Objective: To establish a method to determine aflatoxins B1

G2 in Chinese Materia Medica and pharmaceutical intermediates microbiological contaminated by immunoaffinity column clean-up combined with post-column derivatization and HPLC-FD detection. Method: Using immunoaffinity column clean-up combined with post-column derivatization

the analytical method to detect aflatoxins B1

G2 was established firstly

and then 14 kind of Chinese Materia Medica and pharmaceutical intermediates microbiological contaminated was detected respectively. Result: The method with the great linear concentration range of 0.15-6.0 ng·mL-1 for aflatoxins G2

and 0.5-20.0 μg·L-1for G1

B1 respectively

was stable and accurate. As a result

aflatoxins B1was detected in Chuanxiong Rhizoma and Platycladi Semen in the 14 kind of Chinese Materia Medica

while there were none of aflatoxins in the pharmaceutical intermediates made by Chuanxiong Rhizoma. Conclusion: The method established in this study was specific and accurate for the detection of aflatoxins in Chinese Materia Medica and pharmaceutical intermediates.

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