Objective: To optimize separation and purification process parameters of total saponins from Bolbostemma paniculatum by macroporous resin. Method: With absorption and desorption rate of total saponins as indexes

separation and purification performance of macroporous resin on total saponins from B. paniculatum was compared by static and dynamic test

optimum resin was selected and its purification technology was optimized. Result: HPD-750 macroporous resin showed the best property for purification of total saponins.its optimal process conditions were:diameter-height ratio 1 : 5

the concentration of sample solution 1.6 g·mL-1

maximum sample volume 13 mL

eluted by 1 BV water and 8 BV 95% ethanol

flow rate of adsorption and elution 1 mL·min-1

eluent were collected

ethanol was recovered

under these conditions

the content of total saponins was 63.53% by UV. Conclusion: This optimized process was simple and feasible to separate and purify total saponins from B. paniculatum effectively.