Objective: To optimize purification technology of total saponins

flavonoids and lignins from K-CoxB-JN by macroporous resin. Method: Taking the contents of total saponins

flavonoids and lignins as indexes

adsorption and desorption properties of 10 kinds macroporous resins were compared

based on these

maximum sample volume

sample flow rate and elution solvent were investigated by single-factor test. Result: D101 macroporous resin gave good static-dynamic and adsorption-desorption characteristics for all components

optimized purification technology was:crude drug concentration of alcohol extraction solution 0.5 g ·mL-1

herbs-wet resin 1: 4

adsorption flow rate 2 BV ·h-1

washed with 3 BV distilled water

4 BV of 70% ethanol as elution solvent.Under these conditions

purity of total saponins

total flavonoids and total lignins were enriched from 5.36%

0.3%

2.26% to 53.82%

3.01%

22.87%. Conclusion: This optimum purification technology of K-CoxB-JN was simple

stable and effective with good purification effect

it could be promoted in large-scale production.