CHEN Pei-xin, GAO Ying, LI Wei-min. Optimization of Extraction Technology of Theanine from Tea Stalk by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 39-41.
CHEN Pei-xin, GAO Ying, LI Wei-min. Optimization of Extraction Technology of Theanine from Tea Stalk by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 39-41.DOI:
Objective: To optimize extraction technology of theanine from tea stalk. Method: With the content of theanine as index which was determined by HPLC
ratio of liquid-material
extraction solvent
extraction temperature
extraction time and extraction times were selected as factors
factors and levels were determined by single-factor test; Orthogonal test was used to optimize extraction technology with ratio of liquid-material
extraction temperature and extraction time as factors. Result: Optimum extraction process was:extracted 1 h with 20 times the amount of water at 80 ℃. Conclusion: This optimized technology was stable and reliable with high extraction rate.