Objective: To develop a RP-HPLC method for the quantitative analysis of daurisoline and dauricine

and establish an HPLC-UV fingerprint of Rahizoma Menispermi. Method: The alkaloids were separated on a ZORBAX SB-C18 (4.6 mm×250 mm

5 μm)with gradient elution using acetonitrile and H2O-formic acid (100∶0.5)solvent system and detected at 268 nm. Flow rate was 1.0 mL·min-1 and 10μL was injected every time. Result: The linear range of daurisoline and dauricine was 0.072-2.29 mg·mL-1(r=0.999 6)

0.053-1.70 mg·mL-1(r=0.999 9)

respectively. The average recovery rates of daurisoline and dauricine were 98.7% (RSD 1.5%)

100.6%(RSD 1.9%)

respectively. In the fingerprint

9 common peaks of Rahizoma Menispermi from Chengde and 13 common peaks of Rahizoma Menispermi from other production areas were confirmed. The accuracy and reproducibility of the method of fingerprint were good. The results of similarity analysis were above 0.9 except No.2 was 0.404 of Rahizoma Menispermi from Chengde and No.9 was 0.716 from other production areas. Conclusion: The method can be used scientifically to evaluate the quality of the Rahizoma Menispermi qualitatively and quantitatively.