Objective: To establish a method for the preparation of rhamnocitrin reference substances from Oxytropis falcata. Method: Separated using silica gel column chromatography preliminarily

and then separated and purified rhamnocitrin by Sephdex LH-20 column

thin-layer chromatography and area normalization of HPLC was used to detect purity

spectral analysis identified its structure. Result: The preparation of compounds of rhamnocitrin

had a purity of more than 98%. Conclusion: The preparation method has high efficiency

the reference substance induced has high purity and meet the relevant technical requirements of traditional Chinese medicine chemical reference substance

can be used for mass production of the reference substance used for traditional Chinese medicine qualitative and quantitative analysis.