Objective: To investigate the effect of Daiping granules on the gene expression of Glut4 and IRS-1 in skeletal muscle of rats with type 2 diabetes and explore its possible molecular mechanism in decreasing the blood sugar. Method: The rat type 2 diabetes model was established by feeding with high-fat diets and injecting streptozocin (STZ) into caudal vein. The model rats were randomly divided into model group (M)

Metformine group (Met)

Daiping granules low dose group (DL)

Daiping Granuleshigh dose group (DH)

at the same time normal control group (N) was established. After four weeks of treatment

blood was collected for measurement of fasting blood glucose(FBG)

serum lipid and fasting insulin(FINS)

and insulin sensitivity index(ISI) was calculated.Real-time quantitative PCR technology was employed to measure Glut4 mRNA and IRS-1 mRNA expression of in the skeletal muscle. Result: After 4 weeks of dosing

compared with M group·L-1

ISI:(-6.05±0.29)]

FBG·L-1

DH:(11.21±2.95)mmol·L-1] was decreased significantly(P<0.01) and ISI was increased (P<0.01) in Met group and DH group. Contents of Glut4 mRNA and IRS-1 mRNA in M group were respectively 63.3%

67.8%(P<0.01);compared with M group

contents of Glut4 mRNA were increased 31%(P<0.01)and IRS-1 mRNA were not significantly increased(P>0.05)in Met group;while contents of Glut4 mRNA and IRS-1 mRNA in DH group were

respectively

increased 88.9% (P<0.01)and 82.4%( P<0.01). Conclusion: Daiping granules has a good effect on decreasing FBG in rats with type 2 diabetes

and its mechanism is probably related to increasing insulin sensitivity of the target tissues

enhancing the expressional level of Glut4 mRNA and IRS-1 mRNA in skeletal muscle and improving the taking in and metabolism of blood glucose.