Objective: To optimize preparation technology of inclusion compound of volatile oil from Cnidium monnieri with hydroxypropyl-β-cyclodextrin (HP-β-CD). Method: With utilization rate of volatile oil and yield of inclusion compound as indexes

inclusion compound of volatile oil from C. monnieri with HP-β-CD was optimized by orthogonal design.Quality control of inclusion compound was studied by UV technology

microscopic identification and GC-MS analysis. Result: Optimum inclusion technology was:volatile oil to HP-β-CD 1∶8(mL·g-1)

inclusion time 2.0 h

inlusion temperature 40 ℃.It verified that UV absorption spectrum was the same before and after inclusion

volatile oil had been inclusion by microscopic identification

GC-MS verified that composition in volatile oil was essentially unchanged before and after inclusion. Conclusion: Both of extraction and inclusion technology were reasonable and feasible

it could provide experimental basis for rational use of clinical formulation and development of inclusion.