JIANG Ding-yun, JIANG Chang-xing, XIONG Qing-ping, et al. Optimization of Supercritical COExtraction Technology for Oleanolic acid from [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 20-24.
JIANG Ding-yun, JIANG Chang-xing, XIONG Qing-ping, et al. Optimization of Supercritical COExtraction Technology for Oleanolic acid from [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 20-24.DOI:
Objective: To optimize extraction technology conditions of oleanolic acid from Chaenomeles speciosa by supercritical CO2. Method: HPLC was used to determine the content of oleanolic acid
with average between yield and content of oleanolic acid as index
single factor test and orthogonal test were used to investigate extraction temperature
extraction pressure
separation temperature
separation pressure and kind of carrying agent
and compared with optimized process and documentation process. Result: Optimum extraction technology conditions were:extraction temperature of 45 ℃
extraction pressure of 40 MPa
separation temperature of 30 ℃
separation pressure of 8 MPa and carrying agent of 95% ethanol;Supercritical CO2 extraction was superior to other documentation methods. Conclusion: This optimized technology was feasible and stable
it could be used to promote application of oleanolic acid.