Objective: To optimize separation and purification technology of total flavonoids from Glycyrrhiza uralensis by macroporous adsorption resin. Method: Twelve different types of resin were selected with the amount of absorption and desorption ratio of total flavonoids as indexes

based on this

separation and purification technology of total flavonoids were optimized. Result: Purification effect of AB-8 type macroporous was the best

its optimum technology conditions were:the concentration of sample solution 0.2 g·mL-1

maximum sample quantity 2 mL to 1 mL resin

velocity of absorption 0.5 BV·h-1

pH of sample solution 6

6 BV water to remove impurities at 1 BV·h-1

eluted by 4 BV 70% ethanol at flow rate of 1 BV·h-1.Under this optimized process

purity of total alkaloids in elution was 38%. Conclusion: This optimized process was rational

feasible and suitable for industrial production.