Objective: To establish determination method of in virto release of Brucea javanica oil self-microemulsion. Method: The content of oleic acid from B. javanica oil was determined by GC method

to determine optimum conditions of dissolution determination of B. javanica oil self-microemulsion.Chromatographic conditions were:DB-FFAP capillary column (0.25 mm×30 m

0.25 μm)

column temperature of 205 ℃

temperture of inlet and detector were 220 ℃

sample volume 1 μL. 0.1 mol·L-1 HCl as dissolution medium

hard capsule shell as a carrier

in vitro release of B. javanica oil self-microemulsion drug delivery system was determined by rotating basket method. Result: Cumulative dissolution of B. javanica oil self-microemulsion was 90% in 15 min. Conclusion: This determination method was accurate and reliable

and it could be used for quality control of B. javanica oil self-microemulsion.