Objective: To optimize purification technology for total flavonoids from Gynostemma pentaphyllum by polyamide resin. Method: With the content of total flavonoids as index

UV spectrophotometry was used

adsorption and elution behavior of polyamide resin for total flavonoids was investigated under static and dynamic conditions

and to select optimum technology parameters of purification technology for total flavonoids by polyamide resin. Result: Optimum purification technology parameters were:at room temperature

the concentration of crude drug 0.35 g·mL-1

pH 3.0 and 4 BV 60% ethanol as eluant. Under these conditions

purity of total flavonoids increased from 23.91% to 81.97%

retention rate of total flavonoids in the purified was up to 90.11%. Conclusion: Purified effect of total flavonoids from G. pentaphyllum by polyamide resin was preferable

it was suitable for industrial production.