ZHOU Hai-song, ZHANG Wen-ting, LING Shuang, et al. Comparison of Different Extraction Methods for Antidepressant Components from Lihuan Jieyu Prescription[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(14): 56-58.
ZHOU Hai-song, ZHANG Wen-ting, LING Shuang, et al. Comparison of Different Extraction Methods for Antidepressant Components from Lihuan Jieyu Prescription[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(14): 56-58.DOI:
Objective: To optimize extraction method of antidepressant ingredients from Lihuan Jieyu prescription. Method: Water extraction
ethanol extraction and water-ethanol extraction were used to extract antidepressant active substances from Lihuan Jieyu prescription
optimum extraction method was determined by pharmacodynamics tests
such as forced swimming test
reserpine hypothermia test and open tank test in mice. Result: Compared with model group
water extraction group of Lihuan Jieyu decoction could significantly shortened immobility time of mice in forced swimming test
it showed antidepressant effect.There was significantly difference of water extraction group and model group in reserpine hypothermia test.In open tank test
there was no significant difference of spontaneous activity in all groups. Conclusion: Water extraction method was optimum extraction technology for antidepressant components from Lihuan Jieyu prescription.