Objective: To investigate effect of absorption enhancers on intestinal absorption of silybin and silybin phospholipid complex(SPC). Method: In siturats single pass perfusion model was uesd to investigate absorption of silybin and SPC in duodenum

jejunum

ileum and colon. Result: Silybin and SPC were absorbed in each intestinal segment

especially in jejunum and ileum

and absorption of SPC in each intestinal segments were higher than drug itself.Tween-80 had no significantly absorption enhancement effect on silybin and SPC

glycocholic acid sodium and carbomer only had silghtly promotion absorption

sodium dodecyl sulfate could greatly promote absorption of slilybin while weakly affected SPC;Cremophor RH40 had a strong role in promoting absorption of silybin and SPC. Conclusion: It could improve drug absorption by reasonable modifacaiton of drug structure and selection of suitable absorption enhancers.