Objective: To observe the effect of compound Naokang capsule liquid extract on apoptosis cascade reaction of hippocampus neurons in mouse aging model induced by D-galactose. Method: The mice were randomly divided into 5 groups: normal control group (group E)

D-galactose model group (D group)

high dose group (A group)

middle dose group (B group)

low dose group (C group). 60 mg·kg-1 of D-galactose was sc given to the mice to produce AD model mice daily. Different doses of compound Naokang capsule liquid extract were given to model mice. The expression of apoptosis inducing factor (AIF) of hippocampus neuron

bax

cytochrome C(Cyt C) and Bcl-2 was tested by immunohistochemical staining and Western blotting. Result: The result of Western blotting showed that expression in the model group decreased

while the treatment groups were similar to the control group. The immunohistochemical staining results showed that

compared with control group

the number of AIF positive cells was reduced from 24.7±6.1 to 8.9±1.8

12.6±4.5

13.2±5.7; the number of Bax positive cells was reduced from 47.8±9.7 to 11.2±3.2

29.4±8.2

31.1±6.3

the number of Cyt C positive cells was reduced from 36.7±9.1 to 12.1±3.2

20.2±6.8

25.2±7.1

and the number of Bcl-2 positive cells was increated from 10.4±6.4 to 59.5±7.4

48.6±9.2

32.3±7.0. Conclusion: The apoptosis cascade reaction of hippocampus neurons in mouse aging model induced by D-galactose is obvious

which can be partly improved by compound Naokang capsule liquid extract.