HPLC测定四季三黄片中5种蒽醌类衍生物的含量

冯源; 张德全; 周浓; 姜北; 康小丽

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HPLC测定四季三黄片中5种蒽醌类衍生物的含量

更新时间：2024-01-04

- HPLC测定四季三黄片中5种蒽醌类衍生物的含量
- Five Anthraquinone Derivatives in Siji Sanghuang Tablets Determined by HPLC
- 中国实验方剂学杂志 2012年18卷第7期页码：96-99
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- 纸质出版日期：2012
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冯源, 张德全, 周浓, 等. HPLC测定四季三黄片中5种蒽醌类衍生物的含量[J]. 中国实验方剂学杂志, 2012,18(7):96-99.

FENG Yuan, ZHANG De-quan, ZHOU Nong, et al. Five Anthraquinone Derivatives in Siji Sanghuang Tablets Determined by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(7): 96-99.
冯源, 张德全, 周浓, 等. HPLC测定四季三黄片中5种蒽醌类衍生物的含量[J]. 中国实验方剂学杂志, 2012,18(7):96-99. DOI：

FENG Yuan, ZHANG De-quan, ZHOU Nong, et al. Five Anthraquinone Derivatives in Siji Sanghuang Tablets Determined by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(7): 96-99. DOI：

摘要

目的: 建立四季三黄片中同时测定芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚含量的方法。方法: 色谱柱为Agela Venusil XBP-C18柱(4.6 mm×250 mm

5 μm)

流动相乙腈-0.1%磷酸

梯度洗脱

流速1.0 mL.min-1

检测波长226 nm。结果: 芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚分别在0.040~0.800

0.040~0.800

0.020~0.400 μg呈良好的线性关系

平均回收率(n=6)分别为97.62%

99.48%

99.72%

96.23%

95.68%

RSD 1.62%

1.24%

1.13%

1.19%

1.67%。结论: 所建立的定量方法快速简单

方法可靠

可作为四季三黄片的质量控制方法。

Abstract

Objective: To develop a method for simultaneous determining aloe emodin

rhein

emodin

chrysophanol and physcion in Siji Sanghuang tablets. Method: The method was conducted on Agela Venusil XBP-C18 (4.6 mm×250 mm

5 μm) column. The mobile phase was acetonitrile (A) and 0.1% phosphoric acid (B) with gradient elution. The flow rate was 1.0 mL·min-1 and the detection wavelength was 226 nm. Result: The linear ranges of aloe-emodin

rhein

emodin

chrysophanol and physcione were 0.040-0.800

0.040-0.800

0.020-0.400 μg respectively

the average recoveries (n=6) were 97.62% (RSD 1.62%)

99.48% (RSD 1.24%)

99.72% (RSD 1.13%)

96.23% (RSD 1.19%)

95.68% (RSD 1.67%) respectively. Conclusion: The method is accurate

reliable

repeatable and can be used for the quality control of Siji Sanghuang tablets.

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