Objective: To explore the differences of chemical composition between extracts of Sijunzi Tang prepared by traditional decocting together and single

with fingerprint technology. Method: The analysis was carried out on a Diamonsil C18 (4.6 mm×250 mm

5 μm)with a mobile phase consisting of acetonitrile-water with gradient elution; the flow rate was 0.6 mL·min-1.The column temperature was set at 25 ℃ and UV detection wavelength was set at 203 nm. Result: The ratio of the compositions and its main chemical substances was different between the two extracts.There were 20 peaks in the fingerprint of compatible granules of Sijunzi Tang

and the extract rate of the main chemical substances was higher in extrat decocted together than in extract decocted single. Conclusion: There are some differences in chemical substances and compositions

ratio being different in the extracts traditional of Sijunzi Tang made by decocting together and single

which is provide some scientific reference for the clinical application Sijunzi Tang.