RP-HPLC测定三黄片中黄芩苷和盐酸小檗碱的含量

王西彬; 陈畅; 何希荣; 杨菲; 冯伟红

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RP-HPLC测定三黄片中黄芩苷和盐酸小檗碱的含量

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- RP-HPLC测定三黄片中黄芩苷和盐酸小檗碱的含量
- Determination of Contents of Bacalin and Berberin Hydrochloride in Sanhuang Tablets by RP-HPLC
- 中国实验方剂学杂志 2012年18卷第13期页码：128-130
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- 纸质出版日期：2012
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王西彬, 陈畅, 何希荣, 等. RP-HPLC测定三黄片中黄芩苷和盐酸小檗碱的含量[J]. 中国实验方剂学杂志, 2012,18(13):128-130.

WANG Xi-bin, CHEN Chang, HE Xi-rong, et al. Determination of Contents of Bacalin and Berberin Hydrochloride in Sanhuang Tablets by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 128-130.
王西彬, 陈畅, 何希荣, 等. RP-HPLC测定三黄片中黄芩苷和盐酸小檗碱的含量[J]. 中国实验方剂学杂志, 2012,18(13):128-130. DOI：

WANG Xi-bin, CHEN Chang, HE Xi-rong, et al. Determination of Contents of Bacalin and Berberin Hydrochloride in Sanhuang Tablets by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 128-130. DOI：

摘要

目的: 建立用RP-HPLC法同时测定三黄片中黄芩苷和盐酸小檗碱含量的方法。方法: 采用Accurasil C18色谱柱(4.6 mm× 250 mm

5 μm)

以乙腈-0.2%磷酸水溶液(25:75)为流动相

检测波长为270 nm。结果: 黄芩苷和盐酸小檗碱的线性范围分别为0.043 6~0.436 μg和0.018 5~0.185 μg

回归方程分别为Y=2.79×106X-15622(r=0.9999)和Y=3.98×106X-7512(r=0.9999)加样回收率分别为105.0%

103.0%;RSD分别为1.28%

1.80%。结论: 本法简便

结果准确。

Abstract

Objective: To establish an RP-HPLC method for determination of bacalin and berberin hydrochloride in Sanhuang tablets. Method: The separation was performed on an Accurasil C18(4.6 mm×250 mm

5 μm) column eluted with a mobile phase consisted of acetonitrile-0.2% phosphoric acid solution (25:75) at a flow rate of 1.0 mL·min-1. The column temperature was set at 35℃

and the detection wavelength was set at 270 nm. Result: The linear ranges of bacalin and berberin hydrochloride were 0.043 6-0.436 μg(r=0.9999) and 0.018 5-0.185 μg(r=0.9999)

the average recoveries of bacalin and berberin hydrochloride were 105.0% and 103.0%

and RSD were 1.28% and 1.80% respectively. Conclusion: The method was simple

feasible

and reproducible

and it can be used for the quality control of Sanhuang Tablet.

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