Objective: To research the technologies of Litchi flavonoid separation and the flavonoid constituents. Method: The flavonoid content was detected with rutin as the standard reference by UV absorption

comparisons of the flavonoid extraction effects with ethanol and its acidification solutions were conducted

the technologies of litchi flavonoid separation with macroporous resin were optimized

and the flavonoid constituents of the extraction and separation products were analyzed with HPLC graph. Result: The UV absorption method to detect Litchi flavonoid content was simple and workable; the extraction effect of 70% of ethanol solution was better than its acidification solution

the flavonoid constituent was simpler than the acidification one

and its recovery rate and purity reached (92.5±2.0)% and (28.7±1.8)% respectively. Resin AB-8 had the best effect of flavonoid separation among the 6 macroporous resins

the recovery rate and the purity of the separated product with the eluant of 80% ethanol solution were (86.5 ±2.1)% and (68.6±1.5)% respectively. Conclusion: The technology of extraction with 70% ethanol followed by separation with resin AB-8 to extract and separate Litchi flavonoid was simple and workable.