Objective: To optimize in vitro transdermal test conditions of Xianglian ointment. Method: Drug percutaneous permeability tester was used

the content of total alkaloids in different time

accepting liquid

sampling volume and mouse skin test were determined by UV-spectrophotometry.The cumulative transmittance was calculated to optimize in vitro transdermal test conditions of Xianglian ointment. Result: Optimized in vitro transdermal test conditions were:sampling volume of Xianglian ointment was about 0.1 g

30% ethanol as receiving liquid

rat skin with cutin layer as medium;UV spectrophotometry was used to determine the content of total alkaloid with wavelength 345 nm. Conclusion: This optimized test method was simple

feasible and stable.