Objective: To observe the regulatory effect of Gouteng San on the related indicators on the hippocampal CA1 area in Alzheimer's disease(AD) model rat. Method: AD rat model was established by hippocampal injection of β-amyloid protein(Aβ1-40)

and then divided into high

meddlu and low-dosage

the model and normal control group. Oral administration of Gouteng San (5

2.5

1.25 g·kg-1) was started for 15 constitutive days. The maze behavioral indicators were measured at day 14.After administration of Gouteng San for 1 h

the rats were given anesthesia and cardiac perfusion to obtain the brain

make hippocampal CA1 district biopsy

and immunohistochemical determination was used to assay nerve cells

Aβ

postsynoaptic density-95(PSD-95) positive cells and mean optical density value. Result: After the treatment of Gouteng San

behavioral test results showed that that Gouteng San could improve the learning and memory ability in the rats. The nerve cells in CA1 region

PSD-95 positive cells and average optical density values were higher than model group (P<0.001)

Aβ positive cells and the mean optical density values were lower than the model group (P<0.01)

indicating that Gouteng San could effectively reduce Aβ levels in the brain tissue while increasing PSD-95 positive cells and protecting nerve cells. Conclusion: Gouteng San has a protective effect on the hippocampus area in AD rats.