Objective: To investigate the effects of pachymaran on elimination of jaundice. Method: Fifty male Wistar rats were randomly divided into normal group (10) and alpha-naphthylisothi (ANIT)-induced (40) model group. After 48 h of ANIT treatment

ANIT-treated rats were randomly divided into model group

pachymaran low-dose

medium-dose and high-dose groups (10 rats each group). At the end of the experiment all rats were sacrificed. Liver function

pro-inflammatory and anti-inflammatory cytokines in liver were determined using real-time PCR analysis. Result: Compared with normal group: the acitivity of alanine aminotransferase(ALT)and aspartate aminotransferase (AST) in the serum increased 6.31-fold and 8.31-fold

respectively (P<0.05 or P<0.0.1). The content of total bilirubin (TBil) increased 52.7-fold (P<0.05 or P<0.0.1) in ANIT-treated rats. Compared with the model group

pachymaran high-dose reduced significantly TBil content (P<0.01). Compared with normal group

linterleukin-1 beta(IL-1β) and tumor necrosis factor (TNF-α) mRNA levels increased significantly (P<0.01) and IL-4 was decreased remarkably (P<0.05) in ANIT-treated rats

while the pachymaran high-dose reduced IL-1β and TNF-α mRNA expression (P<0.05 or P<0.1)

elevated liver IL-4 expression(P<0.05). Conclusion: Could be one important mechanism for pachymaran can reduce jaundice by regulating immunologic function in rats.