GAN Xiu-hai, ZHAO Yang, ZHOU Xin, et al. Comparison of the Contents of Quercitin in Various Medicinal Parts of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 100-102.
GAN Xiu-hai, ZHAO Yang, ZHOU Xin, et al. Comparison of the Contents of Quercitin in Various Medicinal Parts of [J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 100-102.DOI:
利用反相高效液相色谱法测定火棘不同部位样品中槲皮素的含量。色谱柱Hypersil ODS (4.6 mm×250 mm
5 μm)
流动相甲醇-0.1%磷酸水溶液(40 :60)
流速1.0 mL·min-1
检测波长360 nm
柱温25 ℃。 结果: 槲皮素在0.048 5~1.552 0 μg线性关系良好
平均回收率为96.18%。 结论: 火棘不同部位中槲皮素的含量差异较大。
Abstract
Objective: To established an RP-HPLC method for determination of quercetin in Pyracantha fortuneana and to compare quercetin contents in different medicinal parts. Method: 90%methanol-25% hydrochloride aqeous solution (4 : 1) was used to extract quercetin and Hypersil ODS (4.6 mm×250 mm
5 μm) column was used to analyze quercetin content with methanol-0.1% phosphoric acid aqueous solution as mobile phases. Flow rate was 1.0 mL·min-1. Detection wavelength was set at 360 nm. Result: The standard curve of quercetin was linear in the range of 0.048 5-1.552 0 μg.The average recovery of quercetin was 98.44%. Conclusion: Quercetin contents in different medicinal parts of P. fortuneana was different.