Objective: To establish a method of fingerprint analysis on Microcos paniculata by HPLC for the quality control. Method: The chromatographic fingerprints were obtained by the Waters Xbridge C18 column (4.6 mm×250 mm

5 μm) with the gradient elution solvent system composed of methanol and 0.05% phosphoric acid. The flow rate was 0.8 mL·min-1; the wavelength was set at 320 nm and the column temperature was maintained at 25 ℃. Result: Twenty main common peaks were selected in the standard fingerprint and three peaks of them were identified as vitexin

isovitexin and narcissoside. There were fifteen samples fingerprints with good similarities with correlation coefficients higher than 0.90 compared with the standard fingerprint in nineteen different samples of M. paniculata

while others were higher than 0.75. Conclusion: The method with good reproducibility is simple and accurate

it can be applied for quality assessment for M. paniculata.