Objective: To study the effects of Siwu decoction with different extracting techniques including water extract

water extract and alcohol precipitate

and 50% ethanol percolation on hematopoietic function in mice with blood deficiency. Method: The mice were randomly divided into six groups: normal gruop

model group

positive group

water extract group

water extract and alcohol precipitate group

50% ethanol percolates group. The Siwu decoction groups were treated with the dosage of 12 g·kg-1

positive group was goven Fufang E-Jiao Jiang 20 mL·kg-1

normal group and model group were given physiological saline

fro 8 days. Except normal group

every group received subcutaneous injection with APH in the second and fifth day with the dosage of 20

40 mg·kg-1 respectively. From the fifth day

every group received peritoneal injection with CTX 40 mg·kg-1 every day for four days. The normal group was given physiological saline. Then

the peripheral hemogram

index of spleen and thymus

blood monomuclear cell(BMC) were detected in different groups. Result: All Siwu decocotion groups could inhance the number of red blood cell(RBC)

white blood cell(WBC)

BMC and the content of HGB

and the effects of water extract group and 50% ethanol percolates group were more obvious (P<0.01) meanwhile Siwu decocotion groups could increase index of spleen and thymus (P<0.01). Conclusion: Siwu decoction can improve the hematopoietic function in mice with blood deficiency

and the water extract group and 50% ethanol percolates group were better than water extract and alcohol precipitate group.