补阳还五汤对大鼠局灶性脑缺血后神经干细胞迁移的影响

苏晓慧; 孔祥英; 庞宗然; 林娜

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补阳还五汤对大鼠局灶性脑缺血后神经干细胞迁移的影响

更新时间：2024-01-04

- 补阳还五汤对大鼠局灶性脑缺血后神经干细胞迁移的影响
- Effect of Buyang Huanwu Decoction on Migration of Neural Stem Cells after Focal Cerebral Ischemia in Rats
- 中国实验方剂学杂志 2012年18卷第6期页码：159-162
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(30873394);中国中医科学院自主选题(ZZ2006105)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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苏晓慧, 孔祥英, 庞宗然, 等. 补阳还五汤对大鼠局灶性脑缺血后神经干细胞迁移的影响[J]. 中国实验方剂学杂志, 2012,18(6):159-162.

SU Xiao-hui, KONG Xiang-ying, PANG Zong-ran, et al. Effect of Buyang Huanwu Decoction on Migration of Neural Stem Cells after Focal Cerebral Ischemia in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 159-162.
苏晓慧, 孔祥英, 庞宗然, 等. 补阳还五汤对大鼠局灶性脑缺血后神经干细胞迁移的影响[J]. 中国实验方剂学杂志, 2012,18(6):159-162. DOI：

SU Xiao-hui, KONG Xiang-ying, PANG Zong-ran, et al. Effect of Buyang Huanwu Decoction on Migration of Neural Stem Cells after Focal Cerebral Ischemia in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 159-162. DOI：

摘要

目的: 观察补阳还五汤对大鼠大脑中动脉阻塞(middle cerebral artery occlusion

MCAO)后神经干细胞(neural stem cells

NSCs)迁移的影响。方法: 采用MCAO模型

缺血90 min后再灌。将造模成功的大鼠分层并随机分组分为模型和补阳还五汤组

24 h后ig补阳还五汤12 g·kg-1

1次/d

连续3周。另16只不阻塞动脉大鼠设为手术组。分别在给药后1

3周进行行为学评分;并在第4天和18天ip 5-溴-2-脱氧尿苷(5-bromo-2-deoxyuridine

BrdU) 50 mg·kg-1

各连续3 d。2

5-三苯基四氮唑(TTC) 染色测量梗死面积;免疫组化检测BrdU阳性细胞;酶联免疫法(ELISA)检测基质细胞衍生因子1(stromal cell derived factor-1

SDF-1)蛋白表达水平。结果: 与假手术组比较

模型组1周和3周神经行为学评分显著升高(P<0.05或 P< 0.01)

脑梗死面积、缺血侧室管膜下区(subventricular zone

SVZ)及纹状体区BrdU阳性细胞数均显著增加(P< 0.05或 P< 0.01)。与模型组相比

补阳还五汤能显著改善大鼠MCAO神经功能缺失症状

药后1

3周神经功能评分为(1.33±0.60)

(0.89±0.58)

(0.50±0.31)分

明显低于模型组的(1.72±0.46)

(1.56±0.51)

(1.89±0.50)分(P<0.01);降低脑梗死面积

药后1

3周分别为(16.1±5.2)%

(10.3±0.40)%

明显低于模型组的(35.2±6.3)%

(29.8±6.9)%

(P<0.01)

提高SVZ和纹状体区BrdU阳性细胞数

药后1周分别为(71.17±5.19)

(83.8±3.83)个

明显高于模型组的(52.17±6.52)

(60.20±6.72)个

药后3周分别为(43.33±6.06)

(54.60±5.77)个

明显高于模型组的(35.83±4.88)

(22.00±3.87)个(P< 0.01);给药3周时显著提高SDF-1表达

3周时为(36.3±2.71)pg·mg-1

明显高于模型组的(28.65±3.47)pg·mg-1(P< 0.05)。结论: 补阳还五汤能促进MCAO大鼠缺血侧NSCs迁移

其可能机制是通过上调SDF-1蛋白的表达水平实现的。

Abstract

Objective: To observe the effect of Buyang Huanwu decoction (BYHWD) on migration of neural stem cells after middle cerebral artery occlusion (MCAO) in rats. Method: Cerebral ischemia/reperfusion model of MCAO was established in rats using the suture method

successful modelings were divided into ischemia model group and BYHWD group randomly. BYHWD (12 g·kg-1) was administered orally 24 h after ischemia once a day for 21 days. Neurological deficit was assessed by neuroethological assessment. The area of cerebral was determined by 2

5-triphenyltetrazolium chloride(TTC) staining. 5-bromo-2-deoxyuridine(BrdU) positive cells were examined by immunohistochemistry

the expression of stromal cell derived factor-1(SDF-1) measured by ELISA. Result: After MCAO the neurological behavioral score

cerebral ischemia area

the number of BrdU-positive cells were significantly increased (P<0.05 or P<0.01). Compared to model group

BYHWD could significantly reduce neurological behavioral score and cerebral ischemia areas(P<0.05 or P<0.01)

enhance the SDF-1 protein level (P<0.05). The number of BrdU-positive cells in subventricular zone(SVZ) and corpus striatum also increased markedly(P<0.05 or P<0.01). Conclusion: BYHWD improves migration of neural stem cells in ischemic penumbra after MCAO

the possible mechanism is related to the increased expression of SDF-1.

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