Objective: To develop a high performance liquid chromatographic method for the determination of harpagide and harpagoside in Zengye Chengqi Koufuye. Method: High performance liquid chromatography was performed on an Eclipse XDB C18(4.6 mm×150 mm

5 μm)with acetonitrile-0.03% phosphoric acid with a gradient elution program as the mobile phase at a flow rate of 1.0 mL·min-1

210 nm as the detection wavelength

the temperature of column was at 30 ℃. Result: harpagide and harpagoside showed good relationship at the range of 0.257-1.284 μg(r=0.999 8

for harpagide)

0.092-0.460 μg(r=1.000 0

for harpagoside)

the average recoveries (n=5) were: 98.74%(RSD 0.58%)

97.15%(RSD 1.02%). Conclusion: The results showed that the method is simple

accurate stable

and repeatable and it is suitable for quality control of Zengye Chengqi Koufuye.