微波协助提取在中药饮片含量测定中的应用(4)——微波法与药典法测定骨碎补中柚皮苷含量比较

方婧; 杨洪军; 付梅红; 张贝贝; 张梦阳; 杨雪燕; 张康丁; 王祝举; 杨岚; 张东

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微波协助提取在中药饮片含量测定中的应用(4)——微波法与药典法测定骨碎补中柚皮苷含量比较

更新时间：2024-01-04

- 微波协助提取在中药饮片含量测定中的应用(4)——微波法与药典法测定骨碎补中柚皮苷含量比较
- Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs (4) Comparison of Determination of Naringin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method
- 中国实验方剂学杂志 2012年18卷第6期页码：75-77
- 作者机构：
- 作者简介：
- 基金信息：
  
  中药生产技术及过程控制技术标准平台项目;"重大新药创制"科技重大专项(2009ZX09308-003);国家科技支撑计划子课题(2006BAI09B07-02)
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- 纸质出版日期：2012
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方婧, 杨洪军, 付梅红, 等. 微波协助提取在中药饮片含量测定中的应用(4)——微波法与药典法测定骨碎补中柚皮苷含量比较[J]. 中国实验方剂学杂志, 2012,18(6):75-77.

FANG Jing, YANG Hong-jun, FU Mei-hong, et al. Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs (4) Comparison of Determination of Naringin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 75-77.
方婧, 杨洪军, 付梅红, 等. 微波协助提取在中药饮片含量测定中的应用(4)——微波法与药典法测定骨碎补中柚皮苷含量比较[J]. 中国实验方剂学杂志, 2012,18(6):75-77. DOI：

FANG Jing, YANG Hong-jun, FU Mei-hong, et al. Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs (4) Comparison of Determination of Naringin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(6): 75-77. DOI：

摘要

目的: 建立微波协助提取法提取骨碎补中有效成分柚皮苷含量测定方法

比较微波协助提取法与药典法提取柚皮苷优势。方法: 采用Dikma Diamonsil C18色谱柱(4.6 mm×150 mm

5 μm)

流动相为甲醇-醋酸-水(35:4:65)

检测波长283 nm

柱温30 ℃

流速1 mL·min-1。结果: 微波提取时间5 min

提取温度120 ℃

提取溶剂50%甲醇。柚皮苷在0.04~0.6 μg呈良好线性关系

r=0.999 9。平均回收率99.51%(n=6)。结论: 微波提取法较药典法提取柚皮苷更简便快速、结果准确

为一种符合环保绿色理念的含量测定方法

可用于检测骨碎补饮片中有效成分柚皮苷含量。

Abstract

Objective: To establish a microwave-assisted extraction method for the extraction of the naringin from Drynaria fortunei. and compare of the determination of naringin from D. fortunei between microwave-assisted extraction and Chinese pharmacopeia method. Method: Dikma Diamonsil C18 column(4.6 mm×150 mm

5 μm)was used. The mobile phase consisted of methanol-acetic acid-water (35: 4: 65). The temperature of column was mainted at 30 ℃. The flow rate was 1.0 mL·min-1 and the detection wavelength was at 283 nm. Result: Microwave extractive time was 5 min

the temperature was 120 ℃

the extraction solvent was 50%methanol. The calibration curve was in good linearity with in the range from 0.04 to 0.6 μg(r=0.999 9). The average recovery was 99.51% (n=6). Conclusion: The method is simple

rapid

as well as precise and reliable

and can be used for the determination of naringin from D. fortunei.

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