整体柱HPLC同时测定白葛胶囊中4种有效成分

王园姬; 周明瑶; 韩省力; 卢闻

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整体柱HPLC同时测定白葛胶囊中4种有效成分

更新时间：2024-01-04

- 整体柱HPLC同时测定白葛胶囊中4种有效成分
- Simultaneous Determination of Four Active Components in Baige Capsules by HPLC and Monolithic Column
- 中国实验方剂学杂志 2012年18卷第11期页码：82-85
- 作者机构：
- 作者简介：
- 基金信息：
  
  陕西省"13115"科技创新工程重大科技专项(2010ZDKG-105)
- DOI：
  中图分类号：
- 纸质出版日期：2012
- 稿件说明：
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王园姬, 周明瑶, 韩省力, 等. 整体柱HPLC同时测定白葛胶囊中4种有效成分[J]. 中国实验方剂学杂志, 2012,18(11):82-85.

WANG Yuan-ji, ZHOU Ming-yao, HAN Sheng-li, et al. Simultaneous Determination of Four Active Components in Baige Capsules by HPLC and Monolithic Column[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 82-85.
王园姬, 周明瑶, 韩省力, 等. 整体柱HPLC同时测定白葛胶囊中4种有效成分[J]. 中国实验方剂学杂志, 2012,18(11):82-85. DOI：

WANG Yuan-ji, ZHOU Ming-yao, HAN Sheng-li, et al. Simultaneous Determination of Four Active Components in Baige Capsules by HPLC and Monolithic Column[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 82-85. DOI：

摘要

目的: 建立一种同时快速测定白葛胶囊中葛根素、大豆苷元、欧前胡素及异欧前胡素含量的方法。方法: 采用C18整体柱( 4.6 mm×50 mm

2 μm)

甲醇(A)-0.1%冰醋酸(B)为流动相

梯度洗脱

流速1.0 mL·min-1

检测波长254 nm

进样体积5 μL

柱温30 ℃。结果: 在建立的色谱条件下

4种有效成分可获得良好的分离

且各自在一定的浓度范围内线性关系良好(r>0.9995)

方法回收率在95%~105%。结论: 所建立的方法简便、快速、可靠

可用于白葛胶囊中多种成分的快速检测。

Abstract

Objective: a method of simultaneous and fast determination the contents of puerarin

daidzein

imperatorin and isoimperatorin in Baige capsules was established. Method: the monolithic C18 column was used to perform the separation

and the mobile phase was methanol (A)-0.1% glacial acetic acid (B) with gradient elution

the flow rate was 1.0 mL·min-1

the detection wavelength was at 254 nm and the injection volume was 5 μL

the column temperature was 30 ℃. Result: these four components could be well separated under the established chromatographic condition

the linear correlation of each component was fine in certain concentration(r>0.999 5)

the recovery was between 95%-105%. Conclusion: the established method was simple

rapid and reliable and could be successfully applied in the active component detection in Baige capsules.

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