Objective: To study the effect of Shenzhu capsules on AP-1 and IL-2 of ERK signal pathway in the gastric cancer mice with spleen-deficiency and explore the molecular mechanism of Shenzhu Caspules in inhibiting the generation of gastric cancer mice with spleen-deficiency. Method: Seventeen mice were randomly selected as normal group. The other mice were administrated by 15 mL·kg-1·d-1Shanxi white venigar on the first day and 10 mL·kg-1·d-1from the second day for continuous 9 days and by 2.8 mg·kg-1·d-1 diethylnitrosamine(DENA) for 110 days to establish gastric cancer mice model with spleen-deficiency.The model mice were divided into model group

Weifuchun group

and 3 doses of Shenzhu capsules groups(440

220 and 110 mg·kg-1·d-1). The mice of treatmental groups were administracted on the 111th day and for continuous 30 days. The general state of animals were observed

the HE staining was used to study whether Shenzhu capsules inhibited the occurance of gastric cancer with spleen-deficiency and the immunohistochemistry method was used to study the influence of Shenzhu capsules on the ERK1/2

AP-1 and IL-2 expression when the experiment was over. Result: The general state and pathological manifestation of Shenzhu capsule groups mice was improved obviously compared with that of model group ones. The Weifuchun and the 440

220 mg·kg-1·d-1Shenzhu capsules could obviously strengthen expression of ERK1/2

AP-1 and IL-2(P<0.05

P<0.01).110 mg·kg-1·d-1Shenzhu capsules could obviously strengthen the expression of ERK1/2(P<0.01) but not obviously strengthen the expression of AP-1 and IL-2. Conclusion: Shenzhu capsules can inhibit the occurance of gastric cancer with spleen-deficiency by inactivating ERK signal pathway.